www.ebiocell.com
| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, ELISA | Phospho-Bag-3 (Tyr457) Rabbit Polyclonal Antibody detects endogenous levels of Bag-3 protein only when phosphorylated on Tyr457. | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 | |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | 62 |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Bag-3 also known as BIS is a 575 amino acid protein characterized by a C-terminal Bag domain and two N-terminal WW domains. This unique structure allows Bag-3 to interact with the ATPase domain of HSP 70, effectively inhibiting HSP 70′s chaperone activity and promoting the release of HSP 70-bound substrates. Such regulatory function maintains cellular protein homeostasis and prevents misfolded protein accumulation, which can lead to cellular stress and apoptosis. Bag-3′s role in cell cycle control and anti-apoptotic properties suggest potential contributions to tumor progression, making Bag-3 an important target for cancer research. |
| Product Image Gallery | |
 Western blot analysis of extracts on K562, Mouse heart cells, using Phospho-Bag-3 (Tyr457) Rabbit Polyclonal Antibody (EAB14501) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
|
