| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, ELISA | 24 | Human, Mouse |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Bag-2 Rabbit Polyclonal Antibody detects endogenous levels of Bag-2 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Bag-2 plays a crucial role in cellular stress responses by functioning as a co-chaperone for HSC 70, a heat shock protein that assists in protein folding and protection against misfolded proteins. This interaction is vital for maintaining cellular homeostasis, particularly under stress conditions, as Bag-2 helps regulate apoptosis and prevent damaged protein accumulation. Bag-2 is an evolutionarily conserved cytoplasmic protein containing putative N-terminal phosphorylation sites, which may influence activity and interactions. Notably, Bag-2 functions as a key component of the HSC 70/CHIP chaperone-dependent ubiquitin ligase complex, where Bag-2 disrupts CHIP-mediated ubiquitylation by interfering with the association between CHIP and ubiquitin-conjugating enzyme, thereby modulating protein degradation pathways. Bag-2′s ability to regulate these processes underscores importance in cellular function and potential implications in various diseases, including cancer and neurodegenerative disorders. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, HCT116 cells, using Bag-2 Rabbit Polyclonal Antibody (EAB13322) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
|
