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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IHC-P, IF/ICC, ELISA | 19 | Human |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB 1:500-2000 IHC-P=1:100-500 IF/ICC=1:100-500 |
|
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | CD99 Mouse Monoclonal Antibody detects endogenous levels of CD99 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
CD99 also known as MIC2 is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. CD99 plays a significant role in lymphocyte aggregation and adhesion. CD99 exists in two forms produced by alternative splicing: the major form promotes cellular adhesion, while the truncated form inhibits this process. This regulation of adhesion is vital, as CD99 modulates the interaction between lymphocytes and the intercellular adhesion molecule-1 (ICAM-1) through lymphocyte function-associated antigen-1 (LFA-1). Notably, overexpression of the truncated form of CD99 leads to a downregulation of LFA-1, which is associated with the Hodgkin′s and Reed-Sternberg (H-RS) phenotype in certain cells, underscoring the importance of CD99 in maintaining proper cell function and morphology. |
| Product Image Gallery | |
 Western blot analysis of extracts on U937, LN18 cells, using CD99 Mouse Monoclonal Antibody (EAB21927) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
Predicted band size: 19 kDa; Observed band size: 28 kDa |
