| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, ELISA | 85 | Human, Mouse |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | CARS Rabbit Polyclonal Antibody detects endogenous levels of CARS protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
CARS also knonw as CYSRS is a class I aminoacyl‑tRNA synthetase that ligates cysteine to its cognate tRNA to ensure translational fidelity in the cytosol. By controlling the supply of charged tRNA. CARS supports global protein synthesis and couples amino acid availability to proteostasis pathways, including stress responses that modulate translation. Perturbation of aminoacyl‑tRNA synthetase activity can alter ribosome dynamics and trigger integrated stress signaling, with downstream effects on cell growth, metabolism, and redox-sensitive processes that depend on cysteine utilization. Dysregulation of tRNA charging and translation quality control is relevant to research on neurodevelopmental and neuromuscular phenotypes, proliferative disorders, and cellular stress adaptation. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela cells, using CARS Rabbit Polyclonal Antibody (EAB11742) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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