| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG2a | Monoclonal | WB, IHC-P, ELISA | 127 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | DDB1 Mouse Monoclonal Antibody detects endogenous levels of DDB1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
DDB1 also known as DDBA is the large subunit (p127) of the heterodimeric DNA damage-binding (DDB) complex while another protein (p48) forms the small subunit. DDB1 plays a crucial role in the cellular response to DNA damage, particularly damage caused by ultraviolet light. DDB1 is essential for the recognition and repair of DNA lesions, specifically cyclobutane pyrimidine dimers, which are formed when DNA is exposed to UV radiation. DDB1′s ability to bind to these damaged sites is vital for initiating the nucleotide excision repair pathway, thereby preventing mutations that could lead to skin cancer and other genetic disorders. Mutations in the DDB2 gene, which encodes the other subunit of the DDB complex, are linked to xeroderma pigmentosum group E, a condition characterized by extreme sensitivity to sunlight and a predisposition to skin malignancies. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, HepG2 cells, using DDB1 Mouse Monoclonal Antibody (EAB21997) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
|
