| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG2b | Monoclonal | WB, IHC-P, IF/ICC, ELISA | 13 | Human, Mouse, Rat, Pig |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:1000-5000 IHC-P=1:100-500 IF/ICC=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | 4E-BP1 Mouse Monoclonal Antibody detects endogenous levels of 4E-BP1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
4E-BP1 also known as EIF4EBP1 is a member of a family of translation repressor proteins. 4E-BP1 plays a crucial role in regulating protein synthesis by acting as a translational repressor. 4E-BP1 binds to eukaryotic translation initiation factor 4E (eIF-4E), preventing formation of the eIF-4F complex essential for translation initiation. Phosphorylation of 4E-BP1 by various kinases, such as S6 kinase p70, MAP kinases, or protein kinase C, leads to dissociation from eIF-4E, promoting translation of mRNAs critical for cell growth and proliferation. This regulatory mechanism is particularly important in cancer biology, where dysregulation of protein synthesis can contribute to tumorigenesis. 4E-BP1 also interacts with 4E-BP2, which modulates eIF-4E activity, highlighting the complexity of translational control in cellular processes. |
| Product Image Gallery | |
 Western blot analysis of extracts on K562, HCT116 cells, using 4E-BP1 Mouse Monoclonal Antibody (EAB21109) at 1:2000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
Predicted band size: 13 kDa; Observed band size: 15-20 kDa |
