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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, ELISA | 43 | Human, Mouse |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 IHC-P=1:50-300 |
|
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | MBD2 Rabbit Polyclonal Antibody detects endogenous levels of MBD2 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
MBD2 belongs to a larger family of methyl-CpG binding proteins, including MBD1, MBD3, MBD4, and MeCP2, which interact with methylated DNA and contribute to gene silencing. MBD proteins show notable expression in somatic tissues, highlighting their significance in tissue-specific gene regulation. MBD2 plays a crucial role in gene expression regulation through DNA methylation, a key epigenetic modification that influences transcriptional activity. MBD2 binds to methylated DNA, particularly at CpG sites, and recruits histone deacetylases, which contribute to transcription repression. This function maintains cellular identity and regulates developmental processes, as DNA methylation pattern abnormalities can lead to various diseases, including cancer. |
| Product Image Gallery | |
 Western blot analysis of extracts on SH-SY5Y cells, using MBD2 Rabbit Polyclonal Antibody (EAB11755) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
 Immunohistochemistry of paraffin-embedded Human colorectal cancer tissue, using MBD2 Rabbit Polyclonal Antibody (EAB11755) at 1:100 dilution.
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