| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, ELISA | 59 | Human, Mouse |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | EYA2 Rabbit Polyclonal Antibody detects endogenous levels of EYA2 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
EYA2 also known as EAB1 is a member of the eyes absent (EYA) family of proteins. EYA2 plays a crucial role in developmental processes, particularly in myogenesis, where Pax3 induces EYA2 in a signaling cascade essential for muscle development. EYA2 features a highly conserved 271 amino acid domain at its carboxy-terminal, which is critical for function as a transcriptional activator, while the amino-terminal region is rich in proline, serine, and threonine residues, contributing to diverse regulatory roles. EYA2 is predominantly expressed in the cytoplasm during late developmental stages in various tissues, including extensor tendons, ligaments, cranial placodes, branchial arches, the central nervous system, and the developing eye. Six proteins facilitate EYA2′s translocation to the nucleus through homeodomain binding, allowing function in connective tissue patterning. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela cells, using EYA2 Rabbit Polyclonal Antibody (EAB11754) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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