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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IHC-P, FC, ELISA | 65 | Human, Mouse, Rat, Monkey |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 IHC-P=1:100-500 FC=1:50-200 |
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| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | eIF2A/CDA02 Mouse Monoclonal Antibody detects endogenous levels of eIF2A/CDA02 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
eIF2A also known as CDA02, MSTP004, MSTP089 is a eukaryotic translation initiation factor that catalyzes the formation of puromycin-sensitive 80 S preinitiation complexes and the poly(U)-directed synthesis of polyphenylalanine at low concentrations of Mg2+. eIF2A is ubiquitously expressed, with highest levels found in heart, brain, pancreas, and placenta, highlighting essential role in cellular metabolism and protein synthesis. eIF2A functions as a translation initiation factor by binding to methionyl-tRNA and directing it to 40S ribosomal subunits, a critical step in early stages of protein synthesis. Unlike eIF2 complex, which operates in a GTP-dependent manner, eIF2A facilitates this process in a codon-dependent manner, underscoring unique regulatory capabilities. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, Jurkat, A431, HepG2, NIH/3T3 cells, using eIF2A/CDA02 Mouse Monoclonal Antibody (EAB21999) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
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