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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG2b | Monoclonal | WB, IHC-P, IF/ICC, FC, ELISA | 69 | Human, Mouse |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 IHC-P=1:100-500 IF/ICC=1:100-500 FC=1:50-200 |
|
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | eIF4B Mouse Monoclonal Antibody detects endogenous levels of eIF4B protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
eIF4B (Eukaryotic translation initiation factor 4B) is a crucial 611 amino acid protein that plays a significant role in the regulation of protein synthesis by facilitating the binding of mRNA to ribosomes, a process essential for the translation of genetic information into functional proteins. Specifically, eIF4B is involved in the assembly of the translation initiation complex by facilitating the binding of mRNA to the ribosome, which is essential for accurate and efficient protein synthesis. eIF4B contains a single RNA recognition motif and interacts with other initiation factors, such as eIF4A, enhancing their activity and promoting the unwinding of mRNA structures that can inhibit translation. The gene encoding eIF4B is located on human chromosome 12, a region rich in genes and significant in various cellular processes. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, A431, A549, Jurkat, HepG2 cells, using eIF4B Mouse Monoclonal Antibody (EAB21929) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
Predicted band size: 69 kDa; Observed band size: 80 kDa |
