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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, ELISA | 46 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 | |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | PHD2 Rabbit Polyclonal Antibody detects endogenous levels of PHD2 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
PHD2 also known as HIFPHD2, EGLN1 functions as a prolyl hydroxylase, catalyzing post-translational modification of hypoxia-inducible factor (HIF) alpha subunits, which regulates gene expression in response to oxygen levels. This modification determines HIF-α protein stability and activity, influencing cellular responses to hypoxia. PHD2′s ability to hydroxylate HIF-α subunits enables cellular adaptation to varying oxygen conditions, making PHD2 essential for angiogenesis, metabolism, and cell survival. PHD2′s role in stabilizing HIF-1α under normoxic conditions maintains cellular function and environmental response capabilities. PDH2 catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. HIF is a transcriptional complex that plays a central role in mammalian oxygen homeostasis. |
| Product Image Gallery | |
 Western blot analysis of extracts on HEK293, Mouse brain cells, using PHD2 Rabbit Polyclonal Antibody (EAB11905) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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