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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF, ELISA | 85 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 IHC-P=1:50-300 IF=1:50-300 |
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| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | PFKM Rabbit Polyclonal Antibody detects endogenous levels of PFKM protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
PFKM also known as PFK-1, PFKA plays a crucial role in glycolysis by catalyzing the conversion of fructose 6-phosphate and ATP into fructose 1,6-bisphosphate, a key regulatory step in glucose metabolism. This enzymatic activity maintains energy homeostasis in cells, particularly during periods of high energy demand. PFKM functions as a tetrameric enzyme composed of different isoforms, including muscle (PFKM), liver (PFKL), and platelet (PFKP) types, each regulated by various metabolites. ATP and citrate inhibit PFKM, signaling sufficient energy levels, while elevated AMP and fructose-2,6-bisphosphate activate PFKM, with fructose-2,6-bisphosphate being produced by the bifunctional enzyme PFK-2. This intricate metabolite regulation of PFKM underscores its importance in metabolic pathways and highlights potential therapeutic interventions in metabolic disorders. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela cells, using PFKM Rabbit Polyclonal Antibody (EAB11762) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
 Immunohistochemical analysis of paraffin-embedded Human brain tissue, using PFKM Rabbit Polyclonal Antibody (EAB11762) at 1:100 dilution.
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