| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, ELISA | 47 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Flotillin-1 Rabbit Polyclonal Antibody detects endogenous levels of Flotillin-1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Flotillin-1 also known as FLOT1 is a member of the band 7.2/stomatin protein family, has a hydrophobic N-terminal region forming a transmembrane domain, integrating into the lipid bilayer. Flotillin-1 plays a crucial role in forming and maintaining lipid rafts, specialized membrane microdomains rich in sphingolipids and cholesterol, essential for signal transduction, membrane trafficking, and molecular sorting. These lipid rafts serve as platforms for assembling signaling complexes, influencing cellular responses to external stimuli. Notably, Flotillin-1 and Flotillin-2 expression levels are independently regulated, with Flotillin-2 being more ubiquitously expressed. Flotillin-1 association with estrogen receptor X (ER-X) facilitates rapid activation of the MAP kinase cascade, highlighting significance in cellular signaling pathways. |
| Product Image Gallery | |
 Western blot analysis of extracts on SW480, HepG2 cells, using Flotillin-1 Rabbit Polyclonal Antibody (EAB11764) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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