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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, ELISA | 79 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 | |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | GFAT1 Rabbit Polyclonal Antibody detects endogenous levels of GFAT1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
GFAT1 also known as GFPT1 is a member of the N-terminal nucleophile class of amidotransferases, converts fructose-6-phosphate into N-acetylglucosamine-6-phosphate. plays a crucial role as the first and rate-limiting enzyme in the hexosamine biosynthesis pathway, which is essential for the synthesis of glycoproteins and glycolipids that are vital for cellular functions. GFAT1 is primarily located in the cytoplasm, where GFAT1 catalyzes the conversion of fructose-6-phosphate into N-acetylglucosamine-6-phosphate, a precursor for the production of UDP-N-acetylglucosamine. This process links glucose metabolism to the regulation of cellular signaling pathways and gene expression, influencing various physiological processes. Dysregulation of GFAT1 activity has been implicated in metabolic disorders, including hyperglycemia-induced insulin resistance, which is a hallmark of type 2 diabetes. GFAT1 serves as not only a critical enzyme in metabolic pathways but also a potential therapeutic target for managing type 2 diabetes and related conditions. |
| Product Image Gallery | |
 Western blot analysis of extracts on 293T cells, using GFAT1 Rabbit Polyclonal Antibody (EAB11771) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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