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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IP, IHC-P, IF/ICC | 25 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:1000-5000 IP=1:10-100 IHC-P=1:100-500 IF/ICC=1:100-500 |
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| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | HPRT Rabbit Monoclonal Antibody detects endogenous levels of HPRT protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
HPRT is a crucial enzyme in purine metabolism, functioning as a purine salvage enzyme that catalyzes the conversion of hypoxanthine and guanine into their respective mononucleotides, inosine monophosphate and guanosine monophosphate. This enzymatic activity is vital for recycling purines, which are essential building blocks for nucleic acids and energy metabolism. HPRT exists as a homotetramer and requires two magnesium ions as cofactors for activity, highlighting the importance of metal ion interactions in function. Deficiencies in HPRT can lead to severe metabolic disorders, including gout and Lesch-Nyhan syndrome, underscoring HPRT′s critical role in maintaining purine homeostasis. |
| Product Image Gallery | |
 Western blot analysis of extracts on HEK293, K562, Mouse brain cells, using HPRT Rabbit Monoclonal Antibody (EAB22050) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
 Immunohistochemistry of paraffin-embedded Human tonsil tissue, using HPRT Rabbit Polyclonal Antibody (EAB22050) at 1:200 dilution.
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