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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IHC-P, FC, ELISA | 102 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 IHC-P=1:100-500 FC=1:50-200 |
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| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Hexokinase II Mouse Monoclonal Antibody detects endogenous levels of Hexokinase II protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Hexokinase II also known as HXK II, HK2 plays a crucial role in glucose metabolism, catalyzing the conversion of glucose to glucose-6-phosphate, which is the first step in the glycolytic pathway. This enzymatic activity is vital for cellular energy production and metabolic regulation, particularly in tissues that rely heavily on glucose, such as adipose and skeletal muscle. Hexokinase II is one of four hexokinase isoenzymes, each with distinct tissue distributions and regulatory mechanisms. While Hexokinase I is expressed in brain, kidney, and heart tissues, Hexokinase II is predominantly found in skeletal muscle and adipose tissue, where Hexokinase II facilitates glucose uptake in response to insulin. The regulation of Hexokinase II activity is essential for maintaining glucose homeostasis, and dysfunction is associated with metabolic disorders, including diabetes. |
| Product Image Gallery | |
 Western blot analysis of extracts on HEK293, Jurkat cells, using Hexokinase II Mouse Monoclonal Antibody (EAB21921) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
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