| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IP, ELISA | 24 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IP=1:50-200 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | MAD2L1 Rabbit Polyclonal Antibody detects endogenous levels of MAD2L1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
MAD2L1 also known as MAD2 is a component of the mitotic spindle checkpoint. MAD2L1 plays a critical role in the regulation of the cell cycle, specifically at the mitotic spindle assembly checkpoint, where MAD2L1 ensures proper chromosome segregation. This checkpoint is vital for preventing aneuploidy, a condition that can lead to tumorigenesis. MAD2L1 is localized to unattached kinetochores during prometaphase, where MAD2L1 acts as a sensor for spindle fiber attachment; upon successful attachment, MAD2L1 disassociates, allowing the cell to proceed to anaphase. Notably, cells with mutations in MAD2L1 fail to arrest during mitosis in response to improper spindle assembly, leading to missegregation of chromosomes and potential cell death. |
| Product Image Gallery | |
 Western blot analysis of extracts on HuvEc, K562, A549 cells, using MAD2L1 Rabbit Polyclonal Antibody (EAB14782) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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