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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IHC-P | 68 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 IHC-P=1:50-200 |
|
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Munc18-1 Rabbit Monoclonal Antibody detects endogenous levels of Munc18-1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Unc18-1 also known as MUNC18, STXBP1 is a syntaxin-binding protein. Unc18-1 protein plays a crucial role in regulating exocytosis by acting as a chaperone that prevents premature interactions between syntaxin and soluble NSF attachment protein receptors, which are essential for vesicle fusion and neurotransmitter release. This regulation is vital for maintaining proper cellular communication and function, particularly in neurons and endocrine cells. Additionally, Unc18-1 undergoes post-translational modifications, including phosphorylation at specific sites such as Ser 313 and Thr 574, which modulate interactions with Syntaxin 1A and influence exocytotic processes. Unc18-1 mRNA expression in various cell types, including RBL-2H3 mast cells and mouse bone marrow-derived mast cells, underscores its importance in immune responses and cellular signaling pathways. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, 293, HepG2 cells, using Munc18-1 Rabbit Monoclonal Antibody (EAB22028) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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