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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IHC-P, IF/ICC, ELISA | 105 | Human |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 IHC-P=1:100-500 IF/ICC=1:100-500 |
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| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | MSH2 Mouse Monoclonal Antibody detects endogenous levels of MSH2 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
MSH2 plays a crucial role in the DNA mismatch repair (MMR) pathway, which is essential for maintaining genomic stability by correcting errors that occur during DNA replication. MSH2 forms a heterodimer with MSH6 or MSH3 to recognize and bind to mismatched DNA, initiating the repair process. This locus is frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E. coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. Additionally, the identification of additional homologs, PMS1 and PMS2, which are also implicated in MMR and mutated in HNPCC patients, underscores MSH2′s significance in cancer biology. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, HEK293, HCT116 cells, using MSH2 Mouse Monoclonal Antibody (EAB22373) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
 Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue, using MSH2 Mouse Monoclonal Antibody (EAB22373) at 1:200 dilution.
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