| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, ELISA | 210 | Human, Mouse, Monkey |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Cleaved-Notch4 (Val1432) Rabbit Polyclonal Antibody detects endogenous levels of Notch4 protein resulting from cleavage adjacent to Val1432. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Notch4 also known as INT3 is a member of the NOTCH family of proteins. Notch4 is a crucial component of the Notch signaling pathway, which is integral to cell communication and plays a significant role in regulating cell fate decisions during development. Notch4 is primarily located in the cell membrane, where Notch4 functions as a receptor that interacts with ligands such as Jagged1, Jagged2, and Delta. Notch4′s membrane localization enables direct cell-to-cell communication, which is essential for processes such as tissue differentiation and development. Aberrant Notch signaling, including Notch4 overexpression, has been implicated in various human tumors, highlighting Notch4′s potential as a therapeutic target. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, K562, SH-SY5Y cells, using Cleaved-Notch4 (Val1432) Rabbit Polyclonal Antibody (EAB14413) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Predicted band size: 210 kDa; Observed band size: 59 kDa |
