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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, ELISA | 78 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 | |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Nicastrin Rabbit Polyclonal Antibody detects endogenous levels of Nicastrin protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Nicastrin also known as NCSTN is a type I transmembrane glycoprotein that is an integral component of the multimeric gamma-secretase complex. Nicastrin plays a crucial role in the assembly and function of the presenilin complex, which is essential for the proteolytic processing of various substrates, including the β-Amyloid precursor protein (βAPP) and Notch. Nicastrin mediates the signaling pathways associated with Notch and GLP-1 and influences the production of the neurotoxic β-Amyloid peptide, a key factor in the pathogenesis of Familial Alzheimer′s disease (FAD). Nicastrin features hydrophilic amino and carboxy-terminal domains, a short hydrophobic transmembrane domain, and potential sites for post-translational modifications such as N-myristoylation and phosphorylation, which may affect stability and interactions within the presenilin complex. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, MCF7 cells, using Nicastrin Rabbit Polyclonal Antibody (EAB14440) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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