www.ebiocell.com
| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, ELISA | 43 | Human, Mouse |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 | |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | NCK1 Rabbit Polyclonal Antibody detects endogenous levels of NCK1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
NCK1 also known as NCKalpha is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. NCK1 plays a crucial role in mediating tyrosine kinase signaling pathways, including those activated by epidermal growth factor (EGF) and platelet-derived growth factor (PDGF). NCK1 is primarily located in the cytoplasm, where NCK1 facilitates the interaction between various signaling molecules, thereby influencing cellular processes such as growth, differentiation, and cytoskeletal dynamics. NCK1′s cellular location enables rapid responses to extracellular signals, allowing for timely modulation of downstream signaling cascades. NCK1 interacts with p21-activated kinase (PAK), a key regulator of the actin cytoskeleton, which underscores NCK1′s role in coordinating cellular responses to growth factors. |
| Product Image Gallery | |
 Western blot analysis of extracts on DU145 cells, using NCK1 Rabbit Polyclonal Antibody (EAB14926) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
|
