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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, ELISA | 74 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 | |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Nopp140 Rabbit Polyclonal Antibody detects endogenous levels of Nopp140 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Nopp140 also known as p130, NOLC1 is a nucleolar phosphoprotein that plays a crucial role in cellular processes by functioning as both a chaperone for protein import and export from the nucleolus and as a transcription factor. Nopp140′s ability to interact with RNA Polymerase and general transcription factor TFIIB highlights its importance in gene expression regulation. Nopp140 is heavily phosphorylated by casein kinase II, which is essential for binding to nuclear localization signals, thereby facilitating nucleolar transport and function. Nopp140 also colocalizes with NAP57, another nucleolar protein, suggesting a collaborative role in nucleolar activities, which are vital for ribosome biogenesis and cellular growth. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, U2OS, MCF7, NIH/3T3 cells, using Nopp140 Rabbit Polyclonal Antibody (EAB11820) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Predicted band size: 74 kDa; Observed band size: 130 kDa |
