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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, ELISA | 58 | Human |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 | |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | PRAME Rabbit Polyclonal Antibody detects endogenous levels of PRAME protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
PRAME is an antigen that is preferentially expressed in human melanomas and that is recognized by cytolytic T lymphocytes. PRAME plays a crucial role in the immune response against tumors, as PRAME is recognized by cytotoxic T lymphocytes, making PRAME a significant target for cancer immunotherapy. Notably, PRAME is expressed at high levels in a wide range of tumors, including melanomas, non-small-cell lung carcinomas, sarcomas, head and neck tumors, and renal carcinomas, while PRAME expression in normal tissues is limited, which enhances PRAME′s potential as a therapeutic target. PRAME′s ability to elicit a strong immune response in tumor cells underscores PRAME′s importance in the development of targeted cancer treatments, as PRAME may help improve the efficacy of immunotherapeutic strategies aimed at harnessing the body′s immune system to fight cancer. |
| Product Image Gallery | |
 Western blot analysis of extracts on HepG2, Mouse liver, Mouse brain cells, using PRAME Rabbit Polyclonal Antibody (EAB11494) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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