| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, ELISA | 140 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | POLG/DNA Polymerase gamma Rabbit Polyclonal Antibody detects endogenous levels of POLG/DNA Polymerase gamma protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
DNA Polymerase gamma also known as POLG is the catalytic subunit of mitochondrial DNA polymerase. DNA Polymerase gamm plays a vital role in the replication of mitochondrial DNA, which is essential for maintaining cellular energy production and overall cellular function. Unlike nuclear DNA, mitochondrial DNA is inherited maternally and is crucial for encoding proteins involved in the electron transport chain, which is responsible for ATP synthesis. Proper functioning of DNA pol gamma is therefore critical, as mutations or deficiencies can lead to mitochondrial diseases, characterized by energy deficits and a range of clinical manifestations. DNA Polymerase gamm is unique among DNA polymerases as the only one specifically required for mitochondrial DNA replication, highlighting DNA Polymerase gamm′s importance in both cellular metabolism and genetic stability. |
| Product Image Gallery | |
 Western blot analysis of extracts on COLO205, HepG2 cells, using POLG/DNA Polymerase gamma Rabbit Polyclonal Antibody (EAB14937) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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