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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, ELISA | 38 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 | |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Ring1B/RNF2 Rabbit Polyclonal Antibody detects endogenous levels of Ring1B/RNF2 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Ring1B also known as RING2, RNF2 is a member of the RING finger family. Ring1B plays a crucial role in gene expression regulation through involvement in Polycomb group (PcG) protein complexes, which maintain silencing of developmental and cell proliferation genes. Ring1B is primarily located in the nucleus, participating in chromatin-associated class II PcG repressive complex 1 (PRC1/hPRC-H) formation. Ring1B protein is essential for Histone H2A monoubiquitination, a modification contributing to transcription repression during development. A RING-type zinc finger domain in Ring1B enables interactions with other proteins, such as Huntington interacting protein 2 (HIP2), facilitating E3 ubiquitin ligase activity. Ring1B protein is implicated in random and imprinted X chromosome inactivation in female mammals, highlighting significance in epigenetic regulation. |
| Product Image Gallery | |
 Western blot analysis of extracts on K562 cells, using Ring1B/RNF2 Rabbit Polyclonal Antibody (EAB11952) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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