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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF, ELISA | 124 | Human, Mouse |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 IHC-P=1:50-300 IF=1:50-300 |
|
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Upf1/Rent1 Rabbit Polyclonal Antibody detects endogenous levels of Upf1/Rent1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Upf1 also known as Rent1, HUPF1 is a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. Upf1 plays a crucial role in nonsense-mediated RNA decay (NMD), which maintains cellular integrity by preventing translation of defective mRNA transcripts that could produce truncated or harmful proteins. This mechanism is particularly important in eukaryotic cells, where detecting and degrading such transcripts helps protect organisms from potential deleterious effects. Rent1, a mammalian ortholog of Upflp, is essential for embryonic viability and features a complex structure including an N-terminal zinc finger-like domain and NTPase domains, classifying Rent1 as a member of the superfamily group I helicase. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, K562 cells, using Upf1/Rent1 Rabbit Polyclonal Antibody (EAB11848) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Predicted band size: 124 kDa; Observed band size: 110 kDa  Immunohistochemistry of paraffin-embedded Human liver cancer tissue, using Upf1/Rent1 Rabbit Polyclonal Antibody (EAB11848) at 1:100 dilution.
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