www.ebiocell.com
| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IHC-P | 45 | Human, Mouse |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 IHC-P=1:50-300 |
|
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | RCC1 Mouse Monoclonal Antibody detects endogenous levels of RCC1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
RCC1 plays a crucial role in the regulation of the small Ras-related protein Ran, which is essential for various cellular processes, including DNA replication and the transport of RNA and proteins through the nuclear pore complex. RCC1 functions as a guanine nucleotide exchange factor, facilitating the conversion of inactive Ran GDP to active Ran GTP, thereby influencing the dynamics of mitosis and nuclear transport. This regulation is particularly important because the balance between Ran GTP and Ran GDP is critical for maintaining proper cellular function and ensuring accurate cell division. The interaction of RCC1 with other proteins, such as Ran GAP1 and Ran BP1, highlights RCC1′s role in a complex regulatory network that governs cellular processes. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, SH-SY5Y, Jurkat cells, using RCC1 Mouse Monoclonal Antibody (EAB22095) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
Predicted band size: 45 kDa; Observed band size: 50 kDa |
