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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF, ELISA | 19 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 IHC-P=1:50-300 IF=1:50-300 |
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| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Skp1 Rabbit Polyclonal Antibody detects endogenous levels of Skp1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Skp1 also known as p19A, OCP2, Skp1 p19 is a component of SCF complexes, which are composed of this protein, cullin 1, a ring-box protein, and one member of the F-box family of proteins. Skp1 plays a crucial role in the regulation of the cell cycle, particularly in the S phase, where Skp1 associates with cyclin A and cyclin-dependent kinase 2 (Cdk2). This interaction is vital for proper progression of the cell cycle, as cyclin A is essential for the transition from G1 phase to S phase, facilitating DNA replication. Skp1 is part of a larger family of regulatory proteins known as Skp proteins, which are involved in the assembly of cyclin-Cdk complexes. Skp1 p19 presence is necessary for the stability and function of the cyclin A-Cdk2 complex, though Skp1 p19 does not directly regulate the activity of these complexes. |
| Product Image Gallery | |
 Western blot analysis of extracts on COS7, NIH/3T3 cells, using Skp1 Rabbit Polyclonal Antibody (EAB11851) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
 Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma tissue, using Skp1 Rabbit Polyclonal Antibody (EAB11851) at 1:100 dilution.
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