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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB | 52 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 | |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-Smad1 (Ser463/Ser465)/Smad5 (Ser463/Ser465)/Smad9 (Ser465/Ser467) Rabbit Monoclonal Antibody detects endogenous levels of Smad1 and Smad5 only when phosphorylated at Ser463/Ser465 and Smad9 when phosphorylated at Ser465/467. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Smad1 also known as MADH1, JV4-1, MADR1 is an intracellular signal transducer and transcriptional modulator involved in the Bone Morphogenetic Protein (BMP) pathway, part of the Transforming Growth Factor-beta (TGF-β) superfamily. Upon activation by BMP receptors, Smad1 is phosphorylated and forms a complex with Smad4, translocating from the cytoplasm to the nucleus. This nuclear localization is crucial because Smad1 regulates the expression of target genes that control cell proliferation, differentiation, and apoptosis. Smad1 function is essential for embryonic development, tissue homeostasis, and bone formation. Dysregulation of Smad1 signaling has been implicated in various diseases, including cancer and fibrosis. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, MCF7, HepG2 cells, using Phospho-Smad1 (Ser463/Ser465)/Smad5 (Ser463/Ser465)/Smad9 (Ser465/Ser467) Rabbit Monoclonal Antibody (EAB22123) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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