www.ebiocell.com
| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IP, IF/ICC | 82 | All |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 IP=1:10-100 IF/ICC=1:50-200 |
|
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | SV40 Large T Antigen Rabbit Monoclonal Antibody detects endogenous levels of SV40 Large T Antigen protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
SV40 T Antigen plays a crucial role in the viral life cycle and is essential for the transformation of host cells, as SV40 T Antigen interacts with key cellular proteins that regulate cell growth and division. Notably, SV40 T Antigen binds to the retinoblastoma (Rb) protein, specifically targeting the "pocket" domain, which is also the binding site for the E2F transcription factor. This interaction leads to the inactivation of Rb, disrupting Rb′s ability to regulate the cell cycle and promoting uncontrolled cell proliferation. SV40 T Antigen′s ability to modulate these critical pathways highlights SV40 T antigen′s importance in understanding viral oncogenesis and the mechanisms of malignant transformation. |
| Product Image Gallery | |
 Western blot analysis of extracts on HL60, A549 cells, using SV40 T Antigen Rabbit Monoclonal Antibody (EAB22302) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Predicted band size: 82 kDa; Observed band size: 90 kDa |
