| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, ELISA | 95 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Sema4D/CD100 Rabbit Polyclonal Antibody detects endogenous levels of Sema4D/CD100 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
CD100 also known as Sema4D is a transmembrane protein belonging to group IV of the semaphorin family. CD100 plays a crucial role in the immune system, being the only member of the semaphorin family expressed in immune tissues. CD100 is vital for T cell proliferation and B cell aggregation, as CD100 regulates B cell responses by counteracting inhibitory signals from CD72, which typically suppresses B cell activity. When CD100 binds to CD72, CD100 induces tyrosine dephosphorylation of CD72, leading to the dissociation of SHP-1, a negative regulator of B cell signaling. CD100 expression is observed in various tissues, including the kidney, heart, lung, and neural tissues, highlighting CD100′s importance in both embryonic and adult development. |
| Product Image Gallery | |
 Western blot analysis of extracts on L929, MCF7 cells, using Sema4D/CD100 Rabbit Polyclonal Antibody (EAB11858) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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