| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, ELISA | 157 | Human |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | SARA Rabbit Polyclonal Antibody detects endogenous levels of SARA protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
SARA also known as NSP, ZFYVE9 a double zinc finger motif-containing protein that participates in the transforming growth factor-beta (TGFB) signalling pathway. SARA plays a crucial role in recruiting Smad proteins to the TGF-beta receptor, ensuring proper subcellular localization of the activated receptor-bound complex. The FYVE domain directs SARA to early endosomal compartments, where SARA interacts with TGF-beta receptors and Smads, facilitating effective signaling pathways. SARA physically interacts with the promyelocytic leukemia (PML) tumor suppressor, which enhances the association and accumulation of SARA and the TGF-beta receptor within early endosomes. SARA can modulate the recruitment of protein phosphatase 1 catalytic subunit (PP1c) to the Smad7-GADD34 complex, influencing the dephosphorylation of the TGF-beta receptor by Smad7, a critical mechanism for regulating negative feedback in TGF-beta signaling. |
