| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, ELISA | 38 | Human, Mouse |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | UCHL5 Rabbit Polyclonal Antibody detects endogenous levels of UCHL5 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
UCHL5 also known as UCH37 is a 329 amino acid protein that plays a crucial role in the 19S regulatory complex of the 26S proteasome, where UCH-L5 functions to edit polyubiquitinated protein substrates. UCH-L5′s ability to rescue ubiquitinated proteins, including those with oncogenic potential, from proteasomal degradation highlights UCH-L5′s importance in cellular regulation and tumor biology. Notably, UCH-L5 interacts with Smad7, leading to a significant upregulation of TGFβ-dependent gene expression through the de-ubiquitination and stabilization of TGFβ receptor I. Given that overexpression of UCH-L5 and other deubiquitinating enzymes has been observed in various cancer cell lines, targeting these proteins may offer promising avenues for cancer therapy. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, 293T cells, using UCHL5 Rabbit Polyclonal Antibody (EAB11947) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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