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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, ELISA | 56 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 IHC-P=1:50-300 |
|
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | PBEF Rabbit Polyclonal Antibody detects endogenous levels of PBEF protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
PBEF also known as Visfatin, NAMPT is a member of the nicotinic acid phosphoribosyltransferase (NAPRTase) family. PBEF plays a crucial role in the biosynthesis of nicotinamide adenine dinucleotide (NAD), a vital coenzyme involved in numerous metabolic processes, including energy production and cellular signaling. PBEF catalyzes the conversion of nicotinamide and 5-phosphoribosyl-1-pyrophosphate into nicotinamide mononucleotide, marking a rate-limiting step in the NAD biosynthesis pathway. This function is particularly important as NAD is essential for the activity of various enzymes, including sirtuins, which are implicated in aging and metabolic regulation. PBEF is predominantly expressed in the cytoplasm and is highly enriched in visceral fat, with expression levels in plasma increasing during obesity development, highlighting its potential role in metabolic disorders. |
| Product Image Gallery | |
 Western blot analysis of extracts on Mouse lung, Mouse kidney cells, using PBEF Rabbit Polyclonal Antibody (EAB11818) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
 Immunohistochemistry of paraffin-embedded Human lung cancer tissue, using PBEF Rabbit Polyclonal Antibody (EAB11818) at 1:100 dilution.
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