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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF, ELISA | 44 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 IHC-P=1:50-300 IF=1:50-300 |
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| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | WTAP Rabbit Polyclonal Antibody detects endogenous levels of WTAP protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
WTAP is a crucial nuclear protein that plays a significant role in regulating mRNA splicing and cell cycle progression, particularly during G2/M transition. WTAP interacts with Wilms′ tumor protein (WT1), known for functioning as a transcriptional repressor, and this interaction maintains proper gene expression and cellular function. WTAP is predominantly located in nuclear speckles, where WTAP binds to the 3′ untranslated region of cyclin A2, enhancing cyclin A2 stability and influencing cell cycle dynamics. WTAP′s role in regulating cyclin A2 levels underscores WTAP′s potential involvement in tumorigenesis, as dysregulation of cell cycle proteins can lead to uncontrolled cell proliferation. WTAP has been shown to inhibit WT1 target gene expression and can impair WT1′s ability to bind DNA, further highlighting WTAP′s regulatory significance in cellular processes. |
| Product Image Gallery | |
 Western blot analysis of extracts on HuvEc cells, using WTAP Rabbit Polyclonal Antibody (EAB11816) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
 Immunohistochemistry of paraffin-embedded Human brain tissue, using WTAP Rabbit Polyclonal Antibody (EAB11816) at 1:100 dilution.
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