| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, ELISA | 97 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Argonaute 2 Rabbit Polyclonal Antibody detects endogenous levels of Argonaute 2 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Argonaute 2 also known as eIF2C2, AGO2 is a member of the Argonaute family of proteins which play a crucial role in RNA interference (RNAi) as integral components of the RNA-inducible silencing complex (RISC) and microRNA (miRNA)-containing ribonucleoprotein particles (miRNPs). Argonaute 2 localizes in cytoplasmic processing bodies (P-bodies), where Argonaute 2 catalyzes mRNA cleavage. This function is vital for the regulation of gene expression, allowing for the degradation of target mRNAs, thereby influencing various cellular processes such as development, differentiation, and response to stress. The interaction of Argonaute 2 with small RNAs, including small interfering RNAs (siRNAs) and miRNAs, facilitates the silencing of target genes, which is essential for maintaining cellular homeostasis and regulating gene expression in response to environmental cues. Argonaute 2 works in concert with other eIF2C proteins, such as eIF2C1 (argonaute 1) and Dicer1, to ensure effective gene silencing through a coordinated mechanism. |
| Product Image Gallery | |
 Western blot analysis of extracts on 293T, MCF7, HepG2 cells, using Argonaute 2 Rabbit Polyclonal Antibody (EAB11960) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Predicted band size: 97 kDa; Observed band size: 90-95 kDa |
