www.ebiocell.com
| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IHC-P, IF, ELISA | 120 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:1000-5000 IHC-P=1:100-500 IHC-P=1:100-500 |
|
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | HIF-1α Mouse Monoclonal Antibody detects endogenous levels of HIF-1α protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
HIF-1α also known as HIF1, MOP1, PASD8, HIF-1alpha is characterized by its multi-domain structure, including the basic helix-loop-helix (bHLH) domain essential for DNA binding, the PAS (Per-Arnt-Sim) domains that facilitate dimerization with HIF-1β (Arnt1), and the oxygen-dependent degradation (ODD) domain that regulates stability under normoxic conditions. HIF-1α contains transactivation domains crucial for recruiting co-activators and initiating transcription of target genes. Understanding the intricate structure of HIF-1α is critical, as this determines the ability to form the active HIF-1 complex, bind to hypoxia-responsive elements (HRE) in the genome, and activate transcription of genes involved in essential processes such as glycolysis, erythropoiesis, and angiogenesis, which are vital for restoring oxygen homeostasis and maintaining cell viability under hypoxic stress. |
| Product Image Gallery | |
 Western blot analysis of extracts on HepG2, Mouse liver cells, using HIF-1α Mouse Monoclonal Antibody (EAB10289) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
|
