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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IP, IHC-P, IF/ICC | 469 | Human |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 IP=1:20-200 IHC-P=1:50-300 IF/ICC=1:50-300 |
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| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | DNA-PKcs Mouse Monoclonal Antibody detects endogenous levels of DNA-PKcs protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
DNA-PKcs also known as XRCC7, DNAPK, PRKDC is a critical component of the DNA-dependent protein kinase complex, DNA-PKcs plays a crucial role in the repair of DNA double-strand breaks (DSBs) and is essential for V(D)J recombination, a process vital for the development of immune diversity. Located in the nucleus, DNA-PKcs forms a heterotrimeric complex with Ku proteins, which are responsible for recognizing and binding to DSBs, thereby facilitating repair processes. Proper functioning of DNA-PKcs is critical, as defects can lead to severe consequences, including increased sensitivity to ionizing radiation and impaired DNA repair mechanisms, which are associated with various genetic disorders and cancers. |
| Product Image Gallery | |
 Western blot analysis of extracts on A549, Hela, K562 cells, using DNA-PKcs Mouse Monoclonal Antibody (EAB22355) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
Predicted band size: 469 kDa; Observed band size: 450 kDa  Immunohistochemical analysis of paraffin-embedded Human Breast cancer tissue, using DNA-PKcs Mouse Monoclonal Antibody (EAB22355) at 1:200 dilution.
 Immunocytochemistry stain of Hela cell, using DNA-PKcs Mouse Monoclonal Antibody (EAB22355) at 1:100 dilution.
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