| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, ELISA | 15 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Atg12 Rabbit Polyclonal Antibody detects endogenous levels of Atg12 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Atg12 also known as APG12 is a 140 amino acid protein that plays a crucial role in the autophagy process, which is essential for cellular homeostasis and the degradation of damaged organelles and proteins. Atg12 is primarily located in the cytoplasm, where Atg12 forms a vital conjugate with APG5, a reaction facilitated by APG7 and Atg10 in the presence of ATP. This conjugation is critical for autophagosome formation, as Atg12 regulates membrane morphology and the overall autophagic process. Atg12′s role in autophagy underscores Atg12′s potential as a therapeutic target in diseases characterized by impaired autophagic function, such as neurodegenerative disorders and cancer. |
| Product Image Gallery | |
 Western blot analysis of extracts on HepG2 cells, using Atg12 Rabbit Polyclonal Antibody (EAB11641) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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