| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, ELISA | 55 | Human, Mouse |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-Atg14 (Ser29) Rabbit Polyclonal Antibody detects endogenous levels of Atg14 protein only when phosphorylated at Ser29. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Atg14 also known as ATG14L is a core component of the class III phosphatidylinositol 3-kinase (PI3KC3) complex I that directs phosphatidylinositol 3-phosphate production at the endoplasmic reticulum to initiate autophagosome nucleation. By coupling Beclin 1–VPS34 signaling to early autophagy membrane dynamics, Atg14 supports autophagosome biogenesis, selective autophagy, and cellular adaptation to nutrient and proteotoxic stress. Disruption of ATG14-dependent autophagy has been linked to altered proteostasis, mitochondrial quality control, and inflammatory signaling, processes frequently studied in cancer biology, neurodegeneration, and metabolic stress models. |
| Product Image Gallery | |
 Western blot analysis of extracts on HuvEc cells treated with Starvation, using Phospho-Atg14 (Ser29) Rabbit Polyclonal Antibody (EAB12147) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide.
Predicted band size: 55 kDa; Observed band size: 65 kDa |
