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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, ELISA | 101 | Human, Mouse |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 |
|
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | ZO-3 Rabbit Polyclonal Antibody detects endogenous levels of ZO-3 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
ZO-3 also known as TJP3 is a member of the membrane-associated guanylate kinase-like (MAGUK) protein family which is characterized by members having multiple PDZ domains, a single SH3 domain, and a single guanylate kinase-like (GUK)-domain. ZO-3 protein plays a crucial role in the formation and maintenance of tight junctions, which are essential for establishing and regulating the paracellular barrier in epithelial and endothelial cells. These tight junctions not only serve as physical barriers to prevent the passage of solutes and pathogens between cells but also play a significant role in cell signaling and maintaining cell polarity. The interaction of ZO-3 with other tight junction proteins, such as occludin and claudins, is vital for proper assembly and function of tight junctions, which in turn influences various physiological processes, including nutrient absorption and immune response. |
| Product Image Gallery | |
 Western blot analysis of extracts on Raw264.7 cells, using ZO-3 Rabbit Polyclonal Antibody (EAB13169) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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