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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, ELISA | 146 | Human, Mouse |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 IHC-P=1:50-300 |
|
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | SF3B1/SAP 155 Rabbit Polyclonal Antibody detects endogenous levels of SF3B1/SAP 155 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
SF3B1 also known as SAP 155 is a crucial component of the SF3b complex, which is associated with the U2 small nuclear ribonucleoprotein (snRNP) and plays an essential role in spliceosome assembly. This complex is vital for the splicing of pre-mRNA, a process that is critical for the proper expression of genes. The interaction of SAP 145 with other spliceosomal proteins, such as SAP 49, SAP 130, and SAP 155, highlights SAP 145′s importance in the formation and stability of the spliceosome. Notably, the direct interaction between SAP 49 and SAP 145 is mediated by the amino-terminus of SAP 49, which contains two RNA-recognition motifs, suggesting this interaction is integral to the complex′s ability to tether U2 snRNP to the branch site during splicing. |
| Product Image Gallery | |
 Western blot analysis of extracts on K562, Jurkat cells, using SF3B1/SAP 155 Rabbit Polyclonal Antibody (EAB13355) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
 Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue, using SF3B1/SAP 155 Rabbit Polyclonal Antibody (EAB13355) at 1:200 dilution.
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