| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, ELISA | 175 | Human, Mouse |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-Scribble (Ser1220) Rabbit Polyclonal Antibody detects endogenous levels of scribble protein only when phosphorylated at Ser1220. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Scribble also known as SCRIB is a protein that was identified as being similar to the Drosophila scribble protein. Scribble plays a crucial role in maintaining epithelial integrity and regulating cell polarity, which is essential for proper tissue architecture and function. Scribble is primarily localized at tight junctions in epithelial cells, where Scribble interacts with other proteins to ensure correct assembly and maintenance of these structures. This localization is vital because tight junctions serve as barriers that control substance passage between cells, thereby influencing processes such as nutrient absorption and pathogen defense. Mutations in Scribble can lead to significant developmental defects and contribute to tumorigenesis, highlighting Scribble′s importance in both normal physiology and disease states. |
| Product Image Gallery | |
 Western blot analysis of extracts on K562 cells treated with UV, using Phospho-Scribble (Ser1220) Rabbit Polyclonal Antibody (EAB13360) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide.
Predicted band size: 175 kDa; Observed band size: 240 kDa |
