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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 68 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
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| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-PLK1 (Ser137) Rabbit Polyclonal Antibody detects endogenous levels of PLK1 protein only when phosphorylated at Ser137. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
PLK1 also known as PLK is a serine/threonine kinase that is closely related to polo and CDC5, genes that are required for passage through mitosis in Drosophila and Saccharomyces, respectively. PLK1 plays a crucial role in cell cycle regulation, particularly during mitosis. PLK1 is predominantly located in the cytoplasm and accumulates during the S and G2 phases of the cell cycle, with levels peaking at the onset of mitosis before rapidly decreasing post-mitosis. PLK1 regulation is vital, as activity is closely linked to cell proliferation and the maintenance of genomic stability. Elevated expression of Plk has been observed in various tumors, suggesting potential as a biomarker for cancer progression and a target for therapeutic intervention. |
| Product Image Gallery | |
 Western blot analysis of extracts on Jurkat cells, using Phospho-PLK1 (Ser137) Rabbit Polyclonal Antibody (EAB12247) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide.
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