| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IF/ICC, ELISA | 45 | Human, Mouse, Monkey |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-Dok-2 (Tyr299) Rabbit Polyclonal Antibody detects endogenous levels of Dok-2 only when phosphorylated at Tyr299. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Dok-2 also known as p56DOK is constitutively tyrosine phosphorylated in hematopoietic progenitors isolated from chronic myelogenous leukemia (CML) patients in the chronic phase. Dok-2 a critical substrate for p210(bcr/abl), a chimeric protein whose presence is associated with CML. Dok-2 plays a crucial role in cellular signaling pathways, particularly in hematopoietic cells, where Dok-2 acts as a negative regulator of growth factor signaling. Dok-2 is primarily located in the cytoplasm, where Dok-2 interacts with various signaling molecules, including the Bcr-Abl fusion protein, which is implicated in chronic myelogenous leukemia. The ability of Dok-2 to modulate signaling pathways is vital for maintaining cellular homeostasis and preventing uncontrolled cell proliferation. |
| Product Image Gallery | |
 Western blot analysis of extracts on Jurkat cells treated with Insulin, using Phospho-Dok-2 (Tyr299) Rabbit Polyclonal Antibody (EAB13577) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide.
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