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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF, ELISA | 25 | Human, Mouse, Monkey |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 IHC-P=1:50-300 IF=1:50-300 |
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| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | MOB1B Rabbit Polyclonal Antibody detects endogenous levels of MOB1B protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
MOB1B is similar to the yeast Mob1 protein. Yeast Mob1 binds Mps1p, a protein kinase essential for spindle pole body duplication and mitotic checkpoint regulation. MOB1B is a conserved MOB family regulator that functions as an essential co-activator of NDR/LATS serine/threonine kinases within the Hippo signaling network. Through these kinase modules, MOB1B contributes to phosphorylation-dependent control of YAP/TAZ transcriptional output, linking cell density cues to proliferation, apoptosis, and organ size regulation. MOB1B also interfaces with cytoskeletal dynamics and centrosome-associated processes that influence mitotic fidelity and cell migration. |
| Product Image Gallery | |
 Western blot analysis of extracts on HEK293, COS7, HuvEc cells, using MOB1B Rabbit Polyclonal Antibody (EAB11974) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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