| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF, ELISA | 72 | Human, Mouse, Rat, Monkey |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-LIMK2 (Ser283) Rabbit Polyclonal Antibody detects endogenous levels of LIMK2 only when phosphorylated at Ser283. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
LIMK2(LIM Kinase 2) is a member of the LIM kinase family. LIMK2 plays a crucial role in regulating actin dynamics, which is essential for various cellular processes such as motility, morphology, and signaling. LIMK2 is primarily located in the cytoplasm and is involved in the phosphorylation of cofilin, a key regulator of actin filament turnover. This phosphorylation inhibits cofilin′s ability to bind to actin, thereby promoting the stabilization of actin filaments and influencing cell shape and movement. LIMK2 functions are vital for processes such as cell migration and differentiation, making LIMK2 an important target for studies related to cancer metastasis and neurodevelopmental disorders. |
| Product Image Gallery | |
 Western blot analysis of extracts on COS7, NIH/3T3 cells, using Phospho-LIMK2 (Ser283) Rabbit Polyclonal Antibody (EAB14522) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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