| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF, ELISA | 35 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | UDG/UNG Rabbit Polyclonal Antibody detects endogenous levels of UDG/UNG protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
UDG(Uracil-DNA glycosylase) also known as UNG plays a critical role in maintaining genomic integrity by excising uracil from DNA, which can arise from misincorporation during DNA replication or from cytosine deamination. This enzymatic activity is essential for preventing mutations that could lead to various diseases, including cancer. UDG operates through a unique mechanism where UDG undergoes a conformational change from an "open" state to a "closed" state upon binding to DNA, which facilitates the cleavage of the glycosidic bond between uracil and deoxyribose. This conformational shift creates a catalytic center that enhances UDG′s ability to flip the uridine nucleotide into the active site for efficient excision. |
| Product Image Gallery | |
 Western blot analysis of extracts on COLO205, HepG2, Rat muscle cells, using UDG/UNG Rabbit Polyclonal Antibody (EAB13480) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
 Immunohistochemical analysis of paraffin-embedded Human tonsil tissue, using UDG/UNG Rabbit Polyclonal Antibody (EAB13480) at 1:200 dilution.
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