| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IF/ICC, ELISA | 80 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Calpain 2 Rabbit Polyclonal Antibody detects endogenous levels of Calpain 2 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Calpain 2 also known as CAPN2 is a crucial intracellular calcium-dependent cysteine protease, plays a significant role in the regulation of cellular processes by cleaving cytoskeletal and submembranous proteins, which is essential for cytoskeletal reorganization and muscle protein degradation. Calpain 2 is primarily located in the cytoplasm and is activated by calcium ions, highlighting its importance in cellular signaling pathways and muscle function. Calpain 2 exists as a heterodimer, consisting of a small regulatory subunit and one of three large catalytic subunits, which include Calpain 1, Calpain 2, and Calpain p94. Calpain activity is tightly regulated by calpastatin, which inhibits both proteolytic activity and membrane binding, with calpastatin existing in tissue and erythrocyte types due to alternative splicing and proteolytic processing. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, A549 cells, using Calpain 2 Rabbit Polyclonal Antibody (EAB13694) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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